二十碳五烯酸的菌種選育及發(fā)酵條件

STRAIN SCREENING AND THE FERMENTATTION CONDITIONS OF EICOSAPENTAENOIC ACID

楊革　徐承水　

摘　要：利用含亞麻子油的斜面培養(yǎng)基連續(xù)傳代和逐漸降低培養(yǎng)溫度,誘導篩選的方法,從大量絲狀真菌中選育到一株產(chǎn)二十碳五烯酸(all-cis-5,8、11、14、17--eicosapentaenoicacid)較高的被孢霉菌(Motierellasp.)SM481.研究得到最適培養(yǎng)基及最適培養(yǎng)條件.在最適培養(yǎng)及產(chǎn)二十碳五烯酸條件下,細胞干重和二十碳五烯酸產(chǎn)量分別為28.8g/L和0.127g/L

關鍵詞：二十碳五烯酸,被孢霉,發(fā)酵調(diào)控

分類號：Q939.97 　文獻標識碼：A

文章編號：1007-3515(2000)03-0366-0370

作者單位：楊革（山東曲阜師范大學生物系曲阜 273165）　

　　　　　徐承水（山東曲阜師范大學生物系曲阜 273165）　

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