黃克服; 劉月英; 鄭忠輝; 黃鴻年; 李少欽; 林君敏
廈門大學(xué)生物學(xué)系; 廈門大學(xué)生物學(xué)系; 廈門大學(xué)生物學(xué)系; 廈門大學(xué)生物學(xué)系; 廈門大學(xué)生物學(xué)系; 廈門大學(xué)生物學(xué)系

【中文摘要】 實驗以甘蔗渣加少量麥麩栽培毛木耳和光木耳,在栽培的不同階段分析測定培養(yǎng)物的的主要化學(xué)組成成分和有關(guān)酶活性的變化。結(jié)果表明,兩種木耳利用栽培底物的能力有明顯差別;在栽培后期,毛木耳和光木耳分別消耗纖維素為74%和43%,半纖維素為78%和61%,木質(zhì)素壽60%和35%。不過它們對這些底物的消耗又具有相似的階段性變化的特點,即在子實體形成期的消耗速率都比菌絲生長期快。培養(yǎng)物的纖維素酶和半纖維素酶的活性也有階段性變化特點。底物降解速率的變化與酶活水平的變化之間有一定對應(yīng)關(guān)系,其中尤以毛木耳較明顯。實驗還分析培養(yǎng)物的過氧化物酶和多酚氧化酶的活性變化,簡要地討論了它們在栽培底物降解中的作用。

【英文摘要】 Auricularia polytricha and A.auricula were separately cultivated on the medium which consisted of sugarcane bagasse mainly.The contents of main chemical constituents and the activities of some enzymes related to the degradation of the substrates in the cultures were determined at different stages during the fungal growth. There were difference in capability to utilize the substrates between the fungi.At the late stage of the cultivation,74% and 43% of cellulose,78% and 61% of hemicellulose,and 60% and 35% of lignin were decomposed by A.polytricha and A.auricula respectively.Both the fungi,however,showed with a similar feature to consume the substrates by stages,i.e.the consumption rates in the fruitbody formation were more rapid than that in the vegetative growth.The activities of cellulase and hemicellulase were changed by stages too.In some extent there existed the positive correlation between the degradation rates of the substrates and the activity levels of the enzymes.Peroxidase a...更多nd polyphenolase also assayed and the roles of the enzymes were discussed briefly.  還原

【中文關(guān)鍵詞】 毛木耳; 光木耳; 甘蔗渣; 栽培; 化學(xué)組分; 降解; 半纖維素酶; 纖維素酶; 多酚氧化酶; 過氧化物酶
【英文關(guān)鍵詞】 Auricularia polytricha; A.auricula; Sugarcane bagasse; Cultivation; Chemical constituents; Degradation; Cellulase; Hemicellulase; Peroxidase; Polyphenolase

【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,1988年06期 【DOI】CNKI:SUN:ZSYJ.0.1988-06-002