金建康; 姚萍; 宋金娣; 王偉明; 丁風珍
江蘇省農(nóng)科院蔬菜所; 江蘇省農(nóng)科院蔬菜所; 江蘇省農(nóng)科院蔬菜所; 江蘇省農(nóng)科院蔬菜所; 江蘇省農(nóng)科院蔬菜所 南京210014; 南京210014; 南京210014; 南京210014; 南京210014

【中文摘要】 雙孢蘑菇的常規(guī)雜交育種由于次級同宗結(jié)合而變得困難。本文研究了蘑菇原生質(zhì)體制備與再生的最佳條件。從異核菌絲出發(fā),制備與再生原生質(zhì)體。根據(jù)自然性狀,推定同核化再生株,栽培驗證同核株,并根據(jù)配對行為確證同核株并分離異核化雜交種。本文認為原生質(zhì)體雜交系統(tǒng)比常規(guī)雜交育種優(yōu)越。

【英文摘要】 The routine hybridization of Agaricus bisporus is difficult for its secondarily homo- thallism.The Optimum preparat on and regeneration of protoplasts of this fungus were studied.Protoplasts were prepared and then regenerated from heterocayotic mycelium, homocaaryodized regenerates were presumed based upon the colonies,and then verified by cultivation fructuation,the authentic homocaryons was confirmed based oa the behavier of pairings,nearly 30 hybrids was eventually obtained.It's suggested that the protoplasty hybridization system is superior to the routine one.

【中文關(guān)鍵詞】 雙孢蘑菇; 原生質(zhì)體; 同核化; 雜交系統(tǒng)
【英文關(guān)鍵詞】 Agaric’us bispocus; Protoplast; Homocaryodization; Hybrdization system

【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,1993年03期 【DOI】CNKI:SUN:ZSYJ.0.1993-03-012