溫磊; 尚德靜; 王偉
遼寧師范大學(xué)生命科學(xué)學(xué)院; 遼寧師范大學(xué)生命科學(xué)學(xué)院; 遼寧師范大學(xué)生命科學(xué)學(xué)院 大連116029; 大連116029; 大連116029

【中文摘要】 靈芝(Ganoderma lucidum)菌絲體通過(guò)生物轉(zhuǎn)化作用富集無(wú)機(jī)硒,分別經(jīng)水、堿水浸提,醇析,去蛋白,脫色后,得到靈芝硒多糖粗提物。再經(jīng)DEAE-Cellu-lose柱層析分離純化后,收集得到的各組分在高效液相色譜上進(jìn)行進(jìn)一步純化,從而得到了10個(gè)不同的硒多糖組分。以K562細(xì)胞進(jìn)行的抗癌試驗(yàn)結(jié)果顯示,SeGLP-2B-1,SeGLP-2B-2和SeGLP-2B-3三種純化硒多糖均有抗癌活性,其中SeGLP-2B-1對(duì)K562細(xì)胞的抑制率最高,達(dá)61.58%。

【英文摘要】 The organic selenium in mycelia of Ganoderma lucidum was enriched by biotransformation.After being extracted in hot water and hot basic water,the extraction were precipitated in ethanol.A series of rough polysaccharide containing selenium can be obtained by removing of protein in and de-coloration.Then the rough extraction passes through the DEAE-cellulose 52,Sephadex G100 and HPLC,10 SeGLPs were obtained.The results of anticancer test in vitro showed that SeGLP-2B-1,SeGLP-2B-2 and SeGLP-2B-3 can inhibit the growth of K562 cells,and the inhibition rate of SeGLP-2B-1,about 61.58%,was highest in all samples.

【中文關(guān)鍵詞】 靈芝硒多糖; 分離純化
【英文關(guān)鍵詞】 SeGLPs; extraction

【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,2005年06期 【DOI】CNKI:SUN:ZSYJ.0.2005-06-017