馬金寶; 沈業(yè)壽; 李峰; 史新強(qiáng); 趙根海; 解要敏
安徽大學(xué)生命科學(xué)學(xué)院生化微生物研究所安徽省生態(tài)工程與生物技術(shù)重點(diǎn)實(shí)驗(yàn)室

【中文摘要】 采用鄰苯三酚自氧化法、Fenton反應(yīng)體系法、DPPH體系法,以抗壞血酸為陽性對(duì)照,對(duì)亮菌多糖-1b(ATPS-1b)清除超氧陰離子自由基(O2-·)、羥自由基(·OH)和二苯代苦味肼基自由基(DPPH·)的效果進(jìn)行了測定。結(jié)果表明ATPS-1b對(duì)O2-·、·OH和DPPH·3種自由基均具有很強(qiáng)的清除作用,且存在良好的量效關(guān)系,隨著ATPS-1b濃度的增大,其清除效果增強(qiáng);清除O2-·、·OH、DPPH·3種自由基的EC50值分別為0.69mg·mL-1、8.73mg·mL-1、2.01mg·mL-1;對(duì)3種自由基的最大清除率分別為86.17%、76.4%、78.52%;ATPS-1b具有顯著的體外抗氧化活性。

【英文摘要】 The effects of polysaccharide-1b from Armillariella tabescens(ATPS-1b) to remove superoxide anion free radical,hydroxyl free radical and DPPH· free radical were determined by the methods of the auto-oxidation of pyrogallol system,Fenton reaction system and DPPH· system. The results showed that ATPS-1b had strong scavenging capacity to O2-·,·OH and DPPH·. Furthermore,scavenging capacity and anthocyanins concentration showed a position of dose-response relationship. The three free radicals had strong scavenging results with the increasing of concenteation. The EC50 of scavenging free radicals were 0.69 mg·mL-1,8.73 mg·mL-1,2.01 mg·mL-1respectively and maximum scavenging rates were 86.17%,76.4%,78.52%. ATPS-1b had significant antioxidant activity in vitro experiments.

【中文關(guān)鍵詞】 亮菌; 多糖; 超氧陰離子自由基; 羥自由基; 二苯代苦味肼基自由基; 抗氧化活性
【英文關(guān)鍵詞】 Armillariella tabescens; Polysaccharide; Superoxide anion free radical; Hydroxyl free radical; DPPH· free radical; Antioxidant activity

【文獻(xiàn)出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2008年06期 【DOI】CNKI:SUN:ZSYJ.0.2008-06-018