徐春燕; 張曉昱; 馬富英; 楊娜
華中科技大學(xué)生命科學(xué)與技術(shù)學(xué)院分子生物物理教育部重點實驗室
【中文摘要】 以糖化率為指標(biāo)對比白腐菌I.lacteus C與G.lucidum E、褐腐菌Fomitopsis sp.IMER2與P.cocos F預(yù)處理玉米秸稈的效果,結(jié)果表明:不同菌株處理效果差異很大,白腐菌預(yù)處理效果優(yōu)于褐腐菌,木質(zhì)纖維素網(wǎng)狀結(jié)構(gòu)中木質(zhì)素去除比纖維素結(jié)晶度降低利于提高糖化率。I.lacteus C預(yù)處理優(yōu)勢明顯,處理30 d糖化率最高,比對照提高135.7%,達(dá)62.0%;G.lucidum E處理后僅20 d樣品糖化率提高,達(dá)42.1%;Fomitopsis sp.IMER2處理后糖化率稍有提高;P.cocos F處理降低糖化率。進(jìn)一步研究發(fā)現(xiàn)I.lacteus C通過改性玉米秸稈提高糖化率:處理30 d可溶性還原糖含量提高287.5%,轉(zhuǎn)化率提高178.3%。
【英文摘要】 In this paper,corn stover saccharification rate was determined to evaluate the effect of biological pretreatment by two kinds of basidiomycetes.Two white rot fungi(I.lacteus C and G.lucidum E) and two brown rot fungi(Fomitopsis sp.IMER2 and P.cocos F) were grown on corn stover for different incubation duration.The results indicated white rot fungi was better than brown rot fungi on enhancing saccharification rate and the effects differed a lot between strains of different varieties and genus,which suggested that,for lignocellulosic biomass,removing lignin was more efficient than reducing cellulose crystallinity for enhancing saccharification rate.Of the four basidiomycetes,I.lacteus C was the best strain for biological pretreatment.The saccharification rate of corn sotver pretreated by I.lacteus C for 30 d reached the highest of 62.0%,which was 135.7% higher than control sample.G.lucidum E enhanced saccharification rate to 42.1% after incubation for 20 d.For brown rot fungi,Fomi-topsis...更多 sp.IMER2 improved the saccharification rates a little,while P.cocos F significantly reduced it.Further study indicated that strain I.lacteus C had the most improved saccharification rate by significantly enhancing soluble reducing sugars contents and transformation rates of corn stover,which were respectively 287.5% and 178.3% higher than control sample.The high sac-charification rate of pretreated corn stover by I.lacteus C had great potential in biological process. 還原
【中文關(guān)鍵詞】 生物預(yù)處理; 白腐菌; 褐腐菌; 木質(zhì)纖維素; 糖化
【英文關(guān)鍵詞】 Biological pretreatment; White rot fungi; Brown rot fungi; Lignocellulose; Saccharification
【基金】國家973項目(項目編號2007CB210200);; 863計劃(項目編號2007AA021301)
【文獻(xiàn)出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2009年06期 【DOI】CNKI:SUN:ZSYJ.0.2009-06-017