許寶奎; 操玉平; 于敏; 沈業(yè)壽; 陸軍
安徽大學(xué)生命科學(xué)學(xué)院安徽省中藥研究與開發(fā)重點實驗室

【中文摘要】 培養(yǎng)大鼠胰島素瘤細胞(INS-1),以四氧嘧啶損傷細胞,培養(yǎng)液中加入不同濃度的AMP-1,MTT法測定細胞存活率,PI熒光染色觀測凋亡細胞密度,流式細胞術(shù)法檢測細胞凋亡率,Western blot法檢測蛋白bcl-2和bax的表達。結(jié)果表明,AMP-1濃度在50 mg·L-1至500 mg·L-1之間,均可提高受四氧嘧啶損傷的INS-1細胞的存活率,其中濃度為400 mg·L-1時存活率最高;凋亡細胞密度,隨AMP-1濃度的增大而降低;AXN+AMP-1組INS-1細胞凋亡率均低于四氧嘧啶組;Western blot法檢測顯示bcl-2蛋白表達量升高,bax蛋白表達量降低。AMP-1對四氧嘧啶誘導(dǎo)大鼠INS-1細胞凋亡具有明顯的拮抗作用,其作用機制可能是通過對線粒體途徑中bcl-2和bax蛋白表達的調(diào)控而抑制了細胞的凋亡。

【英文摘要】 Graded concentrations of AMP-1 were added into the culture medium of INS-1 cells injured by alloxan.The survival rate was measured by MTT assay.The density of apoptotic cells was observed by PI fluorescence staining.The cell apoptosis rate was detected by flow cytometry.The expressions of bcl-2 and bax protein were tested by Western blot.The results showed that the concentrations between 50 mg·L-1 and 500 mg·L-1 of AMP-1 could decrease the injury of INS-1 induced by the AXN, especially at 400 mg·L-1.As the concentration of AMP-1 was increased,the density of the apoptotic cells decreased.The rate of the apoptosis of the AXN+AMP-1 groups were lower than the AXN group.Bcl-2 protein expression was higher with the increased concentration of the AMP-1 while the expression of bax protein was lower.The AMP-1 had the obvious antagonism on the apoptosis of INS-1 cells induced by AXN.The probable mechanism was that AMP-1 could regulate the expression of bcl-2 and bax protein to restrain ...更多the apoptosis of the INS-1 cell.  還原

【中文關(guān)鍵詞】 蜜環(huán)菌菌索多糖; 大鼠胰島素瘤細胞; 細胞凋亡; 拮抗作用
【英文關(guān)鍵詞】 Polysaccharide from the rhizomorph of Armillaria mella(AMP-1); INS-1 cells; Cell apoptosis; Antagonism
【基金】國家自然科學(xué)基金項目(3040066);; 安徽省教育廳自然基金項目(2003kj021;2002kj017)

【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2010年01期 【DOI】CNKI:SUN:ZSYJ.0.2010-01-018