胡國元; 朱蘭寶; 周玉麟
湖北民族學(xué)院應(yīng)用微生物研究所; 華中農(nóng)業(yè)大學(xué)應(yīng)用真菌研究所; 華中農(nóng)業(yè)大學(xué)應(yīng)用真菌研究所 恩施445000 ; 武漢430070 ; 武漢430070

【中文摘要】 采用垂直平板聚丙烯酰胺凝膠電泳,研究不同生長發(fā)育期、不同組織對金針菇[Fla?mmulina velutipes(Fr.)Kar.]黃、白品系酯酶同工酶電泳表型的影響,篩選出不受生長發(fā)育期及常規(guī)培養(yǎng)條件影響的酯酶標(biāo)記區(qū)帶.標(biāo)記區(qū)帶分基本帶和識別帶.酯酶同工酶標(biāo)記區(qū)帶電泳表型顯示出多態(tài)型.利用酯酶同工酶標(biāo)記區(qū)帶分析黃、白金針菇酶譜差異,顯示出黃、白金針菇品系有共同的遺傳基礎(chǔ).

【英文摘要】 Orthogonal slab polyacrylamide gel electrophoresis (OSPAGE) was used to study the effect of different development stages and different tissues on the esterase isozyme electrophoresis phenotype of F. velutipes yellow and white strains. And marker bands of esterase isozyme which were not effected by different development stages and routine culture conditions were screened out. Esterase isozyme marker bands could be divided into primary esterase isozyme marker band which could be obtained from all tested strains, and distinguishing esterase isozyme marker bands which could only be found in some strains. It was proved that OSPAGE phenotype of esterase isozyme marker bands was polymorphic, and that using esterase isozyme marker bands to analyse the zymogram differentiation of F. velutipes yellow and white strains, the common genetic base was found to exist in the tested yellow and white strains.

【中文關(guān)鍵詞】 金針菇; 黃色品系; 白色品系; 酯酶; 同工酶標(biāo)記
【英文關(guān)鍵詞】 Flammulina velutipes; Yellow strain; White strain; Esterase; Isozyme marker
【基金】農(nóng)業(yè)部“八五”重點(diǎn)課題資助.

【文獻(xiàn)出處】 食用菌學(xué)報,Acta Edulis Fungi,編輯部郵箱,1997年04期 【DOI】CNKI:SUN:SYJB.0.1997-04-010