楊焱; 劉艷芳; 張勁松; 周昌艷; 賈薇; 唐慶九
上海市農業(yè)科學院食用菌研究所上海市農業(yè)科學院天然藥用資源研究與開發(fā)中心; 上海市農業(yè)科學院食用菌研究所上海市農業(yè)科學院天然藥用資源研究與開發(fā)中心; 上海市農業(yè)科學院食用菌研究所上海市農業(yè)科學院天然藥用資源研究與開發(fā)中心; 上海市農業(yè)科學院食用菌研究所上海市農業(yè)科學院天然藥用資源研究與開發(fā)中心; 上海市農業(yè)科學院食用菌研究所上海市農業(yè)科學院天然藥用資源研究與開發(fā)中心; 上海市農業(yè)科學院食用菌研究所上海市農業(yè)科學院天然藥用資源研究與開發(fā)中心 201106 ; 201106 ; 201106 ; 201106 ; 201106 ; 201106
【中文摘要】 以菌絲體生物量為主要指標,研究了發(fā)酵培養(yǎng)基中不同碳源、氮源、C/N比、無機元素等營養(yǎng)因子和接種量、裝液量、初始pH、轉速等非營養(yǎng)因素對赤芝G2菌絲生長的影響。研究結果表明,赤芝G2規(guī)模發(fā)酵最適培養(yǎng)基配方為:豆餅粉2%,玉米粉3%,KH2PO40.1%和Mg SO40.05%。發(fā)酵76h,菌絲體生物量最高(1.15g/100mL);發(fā)酵62h,胞內多糖含量最高(22.15%);發(fā)酵60h,胞外多糖含量最高(3.16%)。赤芝G2菌絲深層發(fā)酵的優(yōu)化條件為:起始pH自然,種子液搖瓶裝量40%,規(guī)模培養(yǎng)裝液量20%,接種量10%,發(fā)酵培養(yǎng)時間76h。
【英文摘要】 Taking the mycelial biomass as major evaluation index, the effects of nutritional factors of various carbon and nitrogen sources, the C/N ratio, the inorganic elements and the optimal submerged culture conditions, such as the inoculation rate, the medium capacity, the initial pH and the rotation rate on the mycelial growth of Ganoderma lucidum G2 were investigated. The experimental results showed that the optimum formula of the submerged-culture medium in mass production was soybean cake powder 2%, maize meal 3%, KH_2PO_40.1% and MgSO_40.05%, and that the optimized mode for submerged-culture was as follow: the inoculation rate 10%, the medium capacity for spawn medium 40% and the medium capacity in mass production 20%, the initial pH natural, the rotation rate 150r/min and the submerged-culture time 76h. While the mycelial biomass, the contents of intracellular polysaccharide and the extracellular polysaccharide reached their peaks of 1.15g/100mL, 22.15% and 3.61% after being cultured ...更多for 76h, 62h and 60h respectively. 還原
【中文關鍵詞】 赤芝; 深層發(fā)酵; 營養(yǎng)因子; 優(yōu)化模式
【英文關鍵詞】 Ganoderma lucidum; Submerged-culture; Nutritional factor; Optimized mode
【基金】上海市農委科技興農重點攻關項目"抑瘤靈芝多糖雙高靈芝菌株的篩選"(2001第3-14號)的部分內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2004年03期 【DOI】CNKI:SUN:SYJB.0.2004-03-009