周全; 唐興國; 姚志偉; 高軍偉
武漢生物工程學(xué)院生物工程系; 武漢生物工程學(xué)院生物工程系; 武漢生物工程學(xué)院生物工程系; 武漢生物工程學(xué)院生物工程系 湖北武漢430415; 湖北武漢430415; 湖北武漢430415; 湖北武漢430415

【中文摘要】 通過正交試驗(yàn)篩選出適宜培養(yǎng)姬松茸液體菌種的合成培養(yǎng)基(N6Ⅰ20.0%,N6Ⅱ30.0%,蔗糖5.0%,酵母粉0.3%),以此制備的一級(jí)搖瓶種和二級(jí)搖瓶種,其菌絲體生物量分別為1.48±0.06g/100mL和2.64±0.05g/100mL。栽培試驗(yàn)表明,液體菌種的發(fā)菌時(shí)間比固體菌種縮短13d。

【英文摘要】 A synthetic liquid medium consisting of 20.0% N6Ⅰ(mineral salt solution), 30.0% N6 Ⅱ(vitamin/amino acid supplement), 5.0% sucrose, 0.3% yeast powder was selected by orthogonal testing for culturing Agaricus blazei mycelium. Mycelial yields from shaken primary cultures (50 mL culture medium in 250 mL culture bottles) incubated at 25 ℃ for five days were 1.48±0.06 g/100 mL. Mycelial yields obtained following transfer of mycelium from primary cultures to secondary cultures (70 mL in 250 mL culture bottles) incubated at 25 ℃ for five days were 2.64±0.05 g/100 mL. Colonization of cultivation substrate using the liquid spawn from secondary cultures occurred 13 d earlier compared with normal stock spawn.

【中文關(guān)鍵詞】 姬松茸; N6培養(yǎng)基; 搖瓶培養(yǎng); 液體菌種
【英文關(guān)鍵詞】 Agaricus blaze Murrill; N6 culture medium; Shaking bottle cultivation; Liquid spawn
【基金】武漢市市屬高等學(xué)校2006年度科學(xué)研究項(xiàng)目“巴西蘑菇標(biāo)準(zhǔn)化生產(chǎn)體系的建立”[武教高(2006)5號(hào)]的部分研究內(nèi)容

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2007年02期 【DOI】CNKI:SUN:SYJB.0.2007-02-019