譚著明; DANELL Eric; 申愛(ài)榮; 傅紹春
湖南省林業(yè)科學(xué)院菌根性食用菌研究中心; Museum of Evolution-Botany Section; Uppsala University

【中文摘要】 自1998年始,我們已在湖南、江蘇等地建立了65 ha以上紅汁乳菇—馬尾松種植園。這些林分一般三年后開(kāi)始產(chǎn)菇,年產(chǎn)量可達(dá)675 kg/ha。為了確認(rèn)所產(chǎn)紅汁乳菇子實(shí)體來(lái)源于接種的菌根苗而非自然侵入的孢子,我們進(jìn)行了子實(shí)體、菌根苗菌根的分子研究。首次比較了試驗(yàn)地所產(chǎn)子實(shí)體與紅汁乳菇—馬尾松菌根苗培養(yǎng)缽內(nèi)的菌根的ITS序列。同時(shí),用RAPD法分析了取自于試驗(yàn)地內(nèi)的10個(gè)紅汁乳菇子實(shí)體。結(jié)果表明,10個(gè)子實(shí)體中的9個(gè)與起始菌種CX1相比具有75%96%的相似性。其余一個(gè)及作為外群取自于340 km外的廣西融水的子實(shí)體與起始菌種分別具有61%和37%的相似性。我們認(rèn)為,上述9個(gè)子實(shí)體與起始菌種的相關(guān)性比外群和第10個(gè)子實(shí)體更為緊密。推測(cè)認(rèn)為起始菌株及其子代菌絲形成了上述9個(gè)子實(shí)體,而其余一個(gè)子實(shí)體可能來(lái)源于周邊森林侵入的孢子。

【英文摘要】 Since 1998 we have established more than 65 ha of Lactarius hatsutake-Pinus massoniana plantations in China.The production started three years after plantation,and the annual production may reach 675 kg/ha.To confirm that the fruit bodies originated from our inoculated seedlings and not from mycelia introduced naturally by spores,a molecular study was made.The ITS sequences of fruit bodies from the experimental plot and ectomycorrhizae from Pinus massoniana seedlings in pots were first compared.Then,ten fruit bodies of L.hatsutake collected from the experimental plot were analyzed with RAPD.The results showed that nine of ten fruit bodies in the plot shared 75%～96% of the characters with the original mycelium CX1.One fruit body from the plot and the outgroup fruit body shared 61% and 37% of the characters with the original mycelium.We conclude that nine of ten fruit bodies were more related to each other and to the original mycelium,than to the outgroup or the tenth fruit body.It seems...更多 that the original clone and daughter mycelia of the original clone constituted nine of the ten studied fruit bodies,while one fruit body originated from a mycelium introduced via spores from another forest.  還原

【中文關(guān)鍵詞】 紅汁乳菇; 外生菌根; 栽培; ITS序列; RAPD
【英文關(guān)鍵詞】 Lactarius hatsutake; ectomycorrhizae; cultivation; ITS sequence; RAPD

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2008年03期 【DOI】CNKI:SUN:SYJB.0.2008-03-020