【作者】 田雪蓮;陸偉東;丁偉;卯霞;田雪珊;張軍

【Author】 TIAN Xue-lian1,LU Wei-dong1,DING Wei1,MAO Xia1,TIAN Xue-shan2,ZHANG Jun1(1.Yun-gui Plateau Institute of Biodiversity,Qujing Normal University,Qujing Yunnan 655011;2.Qujing Disease Control Center,Qujing Yunnan 655000)

【機(jī)構(gòu)】 曲靖師范學(xué)院云貴高原生物多樣性研究所;曲靖市疾控中心

【摘要】 通過對(duì)Pm0217F菌株進(jìn)行不同時(shí)間梯度的紫外誘變,得到5株不同的誘變菌株。通過對(duì)5個(gè)誘變株的菌落特征、生長(zhǎng)速率、大孢子千分率、分生孢子不同聚集方式及蟲草菌素含量等生物學(xué)特性比較,結(jié)果顯示,誘變最適時(shí)間為60s,且大孢子比例高、擬青霉型產(chǎn)孢結(jié)構(gòu)比例大的菌株生物學(xué)特性較優(yōu)。

【Abstract】 Ultraviolet radiation to mutate Pm0217F were adopted,and five different strains were obtained at different times of UV mutagenesis.The colony morphology,growth rate,megaspore's permillage,conidiophore of different aggregation methods and content of cordycepin were compared.The results showed that the best time was 60 s,and the strain that had high proportion of megaspore and high proportion of Paecilomyces-type showed the best biological characters.

【關(guān)鍵詞】 蛹蟲草;分生孢子;蟲草菌素;擬青霉型

【Key words】 Cordyceps militaris;Conidiospore;Cordycepin;Paecilomyces-type

【基金】 曲靖師范學(xué)院科技創(chuàng)新團(tuán)隊(duì)基金資助項(xiàng)目

【文獻(xiàn)出處】 中國(guó)食用菌, Edible Fungi of China, 編輯部郵箱 , 2010年05期

【DOI】CNKI:SUN:ZSYJ.0.2010-05-006