【作者】 孫曉紅； 陳明杰； 汪虹； 馮愛萍； 潘迎捷；

【Author】 SUN Xiaohong1,2,3,CHEN Mingjie1,WANG Hong1,FENG Aiping1,PAN Yingjie3 1National Engineering Research Center of Edible Fungi;Key Laboratory of Applied Mycological Resources and Utilization,Ministry of Agriculture;Shanghai Key Laboratory of Agricultural Genetics and Breeding;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China;2College of Life Sciences,Nanjing AgriculturalUniversity,Nanjing,Jiangsu 210095,China;3Shanghai Ocean University,Shanghai 200090,China

【機構】 國家食用菌工程技術研究中心農(nóng)業(yè)部應用真菌資源與利用重點開放實驗室上海市農(nóng)業(yè)遺傳育種重點開放實驗室上海市農(nóng)業(yè)科學院食用菌研究所； 南京農(nóng)業(yè)大學生命技術學院； 上海海洋大學；

【摘要】 根據(jù)草菇(Volvariella volvacea)S-腺苷-L-高半胱氨酸水解酶基因(sahh)全長序列設計兩個嵌套引物,與Takara LA PCR in vitro cloning試劑盒的接頭引物結合進行巢式PCR擴增,得到sahh上游調控序列,通過序列分析發(fā)現(xiàn),該序列含有1個TATA盒和4個CAAT盒。應用MatInspector程序分析sahh上游調控序列,發(fā)現(xiàn)該區(qū)域存在1個耐鹽因子,1個熱激因子,2個DRE元件和2個ABA應答元件。

【Abstract】 An upstream regulatory sequence of a S-adenosyl-L-homocysteine hydrolase gene(sahh) from Volvariella volvacea was amplified by nested PCR using two nested primers designed according to the full-length sequence of sahh and which were connected to the primer linker of the Takara LA PCR in vitro cloning box.The upstream sequence contained one TATA box and four CAAT boxes.MatInspector program analysis revealed one salt resistance factor,one heat shock factor,two drought-response binding factors(DRE)...

【關鍵詞】 S-腺苷-L-高半胱氨酸水解酶基因； 調控序列； 草菇；

【Key words】 Volvariella volvacea； S-adenosyl-L-homocysteine hydrolase gene； regulatory sequence；

【基金】 農(nóng)業(yè)部公益行業(yè)科研專項(編號:nyhyzx07-008)的部分研究內容

【分類號】S646.13